pax6 (+5a) expression in adipose tissue-derived stem cells induces retinal ganglion cells

PAX6 (+5a) Expression in Adipose Tissue-Derived Mesenchymal Stem Cells Induces Differentiation to Retinal Ganglion Cells

Glaucoma remains one of the major causes of blindness in today's world. The progressive field of stem cells proposes an exciting potential for discovering novel therapies. Here, we report the development of an easy and high throughput method for differentiation of retinal ganglion cells (RGCs) and bipolar cells from human adipose tissue-derived mesenchymal stem cells (hADSCs) using PAX6 (+5a) g...

Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells

Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human A...

Differential gene expression by lithium chloride induction of adipose-derived stem cells into neural phenotype cells

Objective(s): Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into neuron-like cells (NLCs).<stro...

Reprogramming by cytosolic extract of human embryonic stem cells improves dopaminergic differentiation potential of human adipose tissue-derived stem cells

The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...

Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells

Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue into Cholinergic-like Cells: In Vitro Study

Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities.  As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells. Methods: In the current study, we set out to investigate the differentiation properties of human adip...